Accurate analysis of collagen in fish tissue is important both as quality control in consumption fish and in optimizing conditions in fish aquaculture. Although collagen is a major component in fish tissue it is a molecule that is difficult to purify and quantitate. This is partly due to the extensive network that is formed by collagen molecules through different types of crosslinking, which makes the collagen molecules insoluble and difficult to extract.
The QuickZyme Total Collagen assay provides an easy and fast method to determine collagen quantities in biological samples. This application note provides guidance for the application of this assay with fish tissue and shows a comparison with HPLC.