The Sensitive Tissue Collagen assay kit is based on the quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. The Sensitive Tissue Collagen assay shows no matrix effect and no need for further dilution of the hydrolysate enabling measuring in small samples with low collagen concentrations, such as liver. The assay is simple and it doesn’t need the drying step following acid hydrolysis, resulting in a fast (<2 hrs) and easy (96-well plate format) assay following the hydrolysis step.

Sensitive Tissue Collagen Assay specifications

• Quantitative measurement of all types of collagen, species independent.
• Samples: tissue homogenates, tissues.
• Colorimetric measurement
• Optimized for tissues with low collagen content such as liver
• No matrix effects as seen in other assays
• Range: 6 to 300 μg/ml. Sensitivity: 2.5 µg/ml
• Uses rat tail collagen as standard
• Ease-of-use: Equivalent to ELISA
• Store at room temperature

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More information

Product sheet: Product sheet Sensitive Tissue Collagen assay
Assay Manual: Manual Sensitive Tissue Collagen assay kit
SDS: assay kit, component 1, 2, 3, 4, 5
Application note 1: How to choose your collagen assay?
Application note 2: How to measure collagen in mouse tissues?
Application note 3: Collagen per protein analysis in fresh, frozen and formalin-fixed tissues
Other information 1: PDF poster “Easy measurement of collagen in tissues”
Other information 2: PDF poster “Collagen analysis in tissues and tissue sections”
Other information 3: PDF poster “Collagen quantification in cell cultures and tissues”
Other information 4: PDF poster “Collagen analysis in tissues: total collagen vs dynamic collagen analysis”
Other information 5: PDF poster “Comparison collagen analysis methods in early fibrosis
Other information 6: PDF poster “Easy and accurate analysis of collagen in NASH fibrosis”